Faculty members have dedicated research laboratory space located within the various buildings on the EVMS campus. These labs, staffed by dedicated principle investigators, research associates, technicians and students are the foundation of the schools research efforts.
Julius O. Nyalwidhe, Ph.D.
Department of Microbiology and Molecular Cell Biology
Leroy T. Canoles Jr. Cancer Research Center
Harry T. Lester Hall 424
651 Colley Avenue
Norfolk, Virginia 23501
Office: (757) 446-5682
Lab: (757) 446-5703
- Biomedical Sciences Program (BSP)
- Molecular Integrative Biosciences (MIB)
- Ph.D. - Philips Universität Marburg, Germany.
- Postdoctoral Fellow - Philips Universität Marburg, Germany.
- Cancer, Infectious Disease and Proteomics/Mass Spectrometry
Tanya C. Burch, Ph.D.
Megan T. Watson, B.S.,
Keywords: Cancer, Cancer Progression and Metastasis, Biomarkers, Prostate Cancer, Health Disparities, Infectious Diseases, Host-Pathogen Interactions, Protein Biochemistry, Protein Post Translational Modifications, Proteomics, Glycomics, Metabolomics, Mass Spectrometry.
Dr. Nyalwidhe’s laboratory focuses on molecular biology, functional genomics and proteomic approaches to study cancer for the purposes of understanding cancer biology as well as to discover clinical biomarkers. The focus is on understanding the molecular events and signaling mechanisms that are involved in cancer progression and disease severity. The objective is to identify and disrupt signaling mechanisms that are involved in cancer progression and severity. The molecules that are involved in these mechanisms are potential diagnostic and prognostic biomarkers for disease and may also provide avenues for targeted cancer drug development. The emphasis is on mechanisms and targets that could explain cancer health disparities between different ethnic groups. Our recent research findings demonstrate the differential regulation of proteins and that their glycosylation patterns closely correlate with prostate cancer disease severity. We are also interested in determining the mechanisms of chemopreventive action of nutraceuticals against cancers. The objective is to identify and characterize the molecular targets and mechanisms of action of known nutraceuticals involved in cancer prevention.
Mechanisms of Host pathogen Interactions
The focus of this project is on understanding the mechanisms of interactions between infectious pathogens and their host cells. A major research focus is the human malaria parasite Plasmodium falciparum, which invades and develops in red blood cells to cause disease pathologies that include severe malarial anemia, nephropathy and cerebral disease. The goal is to define unique and fundamental pathways and mechanisms in the microorganisms as well as human cells that provide molecular insights, targets and therapies in neglected diseases. Additionally, we are also focusing on understanding the mechanisms of pathogenesis of a variety of other infectious agents, and viral-induced carcinomas. The emphasis is on the application of molecular biology, functional and structural proteomics and mass spectrometry techniques to functionally characterize protein post-translational modifications to determine their influence in the progression and outcome of disease.
- Nyalwidhe JO, Betesh LR, Powers TW, Jones EE, White KY, Burch TC, Brooks J, Watson MT, Lance RS, Troyer DA, Semmes OJ, Mehta A, Drake RR.
Increased Bisecting N-Acetylglucosamine and Decreased Branched Chain Glycans of N-linked Glycoproteins in Expressed Prostatic Secretions Associated with Prostate Cancer Progression.
Proteomics Clin Appl. 2013. doi: 10.1002/prca.201200134. PMID: 23775902.
- Burch TC, Watson MT, Nyalwidhe JO.
Variable metastatic potentials correlate with differential plectin and vimentin expression in syngeneic androgen independent prostate cancer cells.
PLoS One. 2013. 8(5):e65005. PMID: 23717685.
- Principe S, Jones EE, Kim Y, Sinha A, Nyalwidhe JO, Brooks J, Semmes OJ, Troyer DA, Lance RS, Kislinger T, Drake RR.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
Proteomics. 2013. 13(10-11):1667-71. PMID: 23533145.
- Nyalwidhe J, Burch T, Bocca S, Cazares L, Green-Mitchell S, Cooke M, Birdsall P, Basu G, Semmes OJ, Oehninger S.
The search for biomarkers of human embryo developmental potential in IVF: a comprehensive proteomic approach.
Mol Hum Reprod. 2013, 19(4):250-63. PMID: 23247814.
- Kim Y, Ignatchenko V, Yao CQ, Kalatskaya I, Nyalwidhe JO, Lance RS, Gramolini AO, Troyer DA, Stein LD, Boutros PC, Medin JA, Semmes OJ, Drake RR, Kislinger T.
Identification of differentially expressed proteins in direct expressed prostatic secretions of men with organ-confined versus extracapsular prostate cancer.
Mol Cell Proteomics. 2012, 11(12):1870-84. PMID: 22986220.
- Sharp JA, Echague CG, Hair PS, Ward MD, Nyalwidhe JO, Geoghegan JA, Foster TJ, Cunnion KM.
Staphylococcus aureus surface protein SdrE binds complement regulator factor H as an immune evasion tactic.
PLoS One. 2012, 7(5):e38407. PMID: 22675461.
- Hair PS, Echague CG, Rohn RD, Krishna NK, Nyalwidhe JO, Cunnion KM.
Hyperglycemic conditions inhibit C3-mediated immunologic control of Staphylococcus aureus.
J Transl Med. 2012, 5:10:35. PMID: 22390383.
- Principe S, Kim Y, Fontana S, Ignatchenko V, Nyalwidhe JO, Lance RS, Troyer DA, Alessandro R, Semmes OJ, Kislinger T, Drake RR, Medin JA.
Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine.
J Proteome Res. 2012,11(4):2386-96. PMID: 22339264.
- Hair PS, Wagner SM, Friederich PT, Drake RR, Nyalwidhe JO, Cunnion KM.
Complement regulator C4BP binds to Staphylococcus aureus and decreases opsonization.
Mol Immunol. 2012, 50(4):253-61. PMID: 22333221.
- Green-Mitchell SM, Cazares LH, Semmes OJ, Nadler JL, Nyalwidhe JO.
On-tissue identification of insulin: in situ reduction coupled with mass spectrometry imaging.
Proteomics Clin Appl. 2011, 5(7-8):448-53. PMID: 21656913.
- Fernandez Falcon MF, Echague CG, Hair PS, Nyalwidhe JO, Cunnion KM.
Protease inhibitors decrease IgG shedding from Staphylococcus aureus, increasing complement activation and phagocytosis efficiency.
J Med Microbiol. 2011, 60(Pt 10):1415-22. PMID: 21636671.
- Yang L, Nyalwidhe JO, Guo S, Drake RR, Semmes OJ.
Targeted identification of metastasis-associated cell-surface sialoglycoproteins in prostate cancer.
Mol Cell Proteomics. 2011, 10(6):M110.007294. PMID: 21447706.
- Gatlin CL, White KY, Tracy MB, Wilkins CE, Semmes OJ, Nyalwidhe JO, Drake RR, Malyarenko DI.
Enhancement in MALDI-TOF MS analysis of the low molecular weight human serum proteome.
J Mass Spectrom. 2011, 46(1):85-9. PMID: 21190259.
- Belgnaoui SM, Fryrear KA, Nyalwidhe JO, Guo X, Semmes OJ.
The viral oncoprotein tax sequesters DNA damage response factors by tethering MDC1 to chromatin.
J Biol Chem. 2010, 285(43):32897-905. PMID: 20729195.
- Gronemus JQ, Hair PS, Crawford KB, Nyalwidhe JO, Cunnion KM, Krishna NK.
Potent inhibition of the classical pathway of complement by a novel C1q-binding peptide derived from the human astrovirus coat protein.
Mol Immunol. 2010, 48(1-3):305-13. PMID: 20728940.
- Guo X, Ward MD, Tiedebohl JB, Oden YM, Nyalwidhe JO, Semmes OJ.
Interdependent phosphorylation within the kinase domain T-loop Regulates CHK2 activity.
J Biol Chem. 2010, 285(43):33348-57. PMID: 20713355.
- Azimzadeh O, Sow C, Gèze M, Nyalwidhe JO, Florent I.
Plasmodium falciparum PfA-M1 aminopeptidase is trafficked via the parasitophorous vacuole and marginally delivered to the food vacuole.
Malar J. 2010, 30; 9:189. PMID: 20591164.
- Drake RR, Elschenbroich S, Lopez-Perez O, Kim Y, Ignatchenko V, Ignatchenko A, Nyalwidhe JO, Basu G, Wilkins CE, Gjurich B, Lance RS, Semmes OJ, Medin JA, Kislinger T.
In-depth proteomic analyses of direct expressed prostatic secretions.
J Proteome Res. 2010, 9(5):2109-16. PMID: 20334419.
- Chao TC, Kalinowski J, Nyalwidhe J, Hansmeier N.
Comprehensive proteome profiling of the Fe(III)-reducing myxobacterium Anaeromyxobacter dehalogenans 2CP-C during growth with fumarate and ferric citrate.
Proteomics. 2010, 10(8):1673-84. PMID: 20162560.
- Britten RA, Mitchell S, Johnson AM, Singletary SJ, Keeney SK, Nyalwidhe JO, Karbassi ID, Lonart G, Sanford LD, Drake RR.
The identification of serum biomarkers of high-let radiation exposure and biological sequelae.
Health Phys. 2010, 98(2):196-203. PMID: 20065683.
- Cazares LH, Troyer D, Mendrinos S, Lance RA, Nyalwidhe JO, Beydoun HA, Clements MA, Drake RR, Semmes OJ.
Imaging mass spectrometry of a specific fragment of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 2 discriminates cancer from uninvolved prostate tissue.
Clin Cancer Res. 2009, 15(17):5541-51. PMID: 19690195.
- Karbassi ID, Nyalwidhe JO, Wilkins CE, Cazares LH, Lance RS, Semmes OJ, Drake RR.
Proteomic expression profiling and identification of serum proteins using trypsin beads with MALDI-TOF/TOF.
J Proteome Res. 2009, 8(9):4182-92. PMID: 19603828.
- Schaub NP, Jones KJ, Nyalwidhe JO, Cazares LH, Karbassi ID, Semmes OJ, Feliberti EC, Perry RR, Drake RR.
Serum proteomic biomarker discovery reflective of stage and obesity in breast cancer patients.
J Am Coll Surg. 2009, 208(5):970-8; discussion 978-80. PMID: 19476873.
- Drake RR, White KY, Fuller TW, Igwe E, Clements MA, Nyalwidhe JO, Given RW, Lance RS, Semmes OJ.
Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease.
J Proteomics. 2009, 72(6):907-17. PMID: 19457353.
- White KY, Rodemich L, Nyalwidhe JO, Comunale MA, Clements MA, Lance RS, Schellhammer PF, Mehta AS, Semmes OJ, Drake RR.
Glycomic characterization of prostate-specific antigen and prostatic acid phosphatase in prostate cancer and benign disease seminal plasma fluids.
J Proteome Res. 2009, 8(2):620-30. PMID: 19128049.
- Bolte K, Kawach O, Prechtl J, Gruenheit N, Nyalwidhe J, Maier UG.
Complementation of a phycocyanin-bilin lyase from Synechocystis sp. PCC 6803 with a nucleomorph-encoded open reading frame from the cryptophyte Guillardia theta.
BMC Plant Biol. 2008, 6; 8:56. PMID: 18485196.
- Jones D, Nyalwidhe J, Tetley L, Barrett MP.
McArthur revisited: fluorescence microscopes for field diagnostics.
Trends Parasitol. 2007, 23(10):468-9. PMID: 17826338.
- Azim-Zadeh O, Hillebrecht A, Linne U, Marahiel MA, Klebe G, Lingelbach K, Nyalwidhe J.
Use of biotin derivatives to probe conformational changes in proteins.
J Biol Chem. 2007, 282(30):21609-17. PMID: 17545162.
- Shamseldin A, Nyalwidhe J, Werner D.
A proteomic approach towards the analysis of salt tolerance in Rhizobium etli and Sinorhizobium meliloti strains.
Curr Microbiol. 2006 52(5):333-9. PMID: 16604415.
- Nyalwidhe J, Lingelbach K.
Proteases and chaperones are the most abundant proteins in the parasitophorous vacuole of Plasmodium falciparum-infected erythrocytes.
Proteomics. 2006, 6(5):1563-73. PMID: 16470785.
- Nyalwidhe J, Maier UG, Lingelbach K.
Intracellular parasitism: cell biological adaptations of parasitic protozoa to a life inside cells.
Zoology (Jena). 2003,106(4):341-8. PMID: 16351918.
- Baumeister S, Endermann T, Charpian S, Nyalwidhe J, Duranton C, Huber S, Kirk K, Lang F, Lingelbach K.
A biotin derivative blocks parasite induced novel permeation pathways in Plasmodium falciparum-infected erythrocytes.
Mol Biochem Parasitol. 2003 132(1):35-45. PMID: 14563535.
- Nyalwidhe J, Baumeister S, Hibbs AR, Tawill S, Papakrivos J, Volker U, Lingelbach K.
A nonpermeant biotin derivative gains access to the parasitophorous vacuole in Plasmodium falciparum-infected erythrocytes permeabilized with streptolysin O.
J Biol Chem. 2002, 18;277(42):40005-1. PMID: 12186876.
- Khan B, Omar S, Kanyara JN, Warren-Perry M, Nyalwidhe J, Peterson DS, Wellems T, Kaniaru S, Gitonga J, Mulaa FJ, Koech DK.
Antifolate drug resistance and point mutations in Plasmodium falciparum in Kenya.
Trans R Soc Trop Med Hyg. 1997, (4):456-60. PMID: 9373654.
|Nyalwidhe J, Baumeister S, and Lingelbach K.
Surface biotinylation of infected erythrocytes:
In Methods in Malaria Research 5th Edition. (2008).
Ljungström, I, Perlmann, H., Schlichtherle, M., Scherf, A and Wahlgren, M (Editors).
|Semmes OJ, Belgnaoui MS, Mitchell R and Nyalwidhe J0.
Application of proteomics to HTLV-1: Understanding pathogenesis and enhancing diagnostics:
In Recent Advances in Human Retroviruses: principles of replication and Pathogenesis. (2010).
Lever AML, Kuan-Teh, J and Berkhout B. (Editors).
|Semmes OJ, Drake RR and Nyalwidhe J0.
Mass Spectrometry: Principles and Applications in Cancer Diagnosis and Management:
In Principles of Molecular Diagnostics and Personalized Cancer Medicine.
- Syncollin, pancreatic triacylglycerol lipase, and other biomarkers for diabetes
WO 2013106851 A2
Inventors: Nadler, JL., Green-Mitchell, SM., and Nyalwidhe, J.O.