Dianne C. Daniel, Ph.D.
Assistant Professor

Adjunct Assistant Professor
Department of Pathology
Mount Sinai School of Medicine
New York, NY

Lewis Hall, #3152
Office: (757) 446-5684
Email: danielDC@evms.edu

Biomedical Sciences Program Track: Molecular Integrative Biosciences (MIB)

Education

• B.S., Lynchburg College, Lynchburg, VA
• M.S., Longwood College, Farmville, VA
• Ph.D., City University of New York, New York, NY
• Postdoctoral Training, Mount Sinai School of Medicine and The Rockefeller University, New York, NY

Research Interests

MCM8, helicase, breast cancer, BRCA1, BRCA2, tumor suppressor, breast organoid, DNA damage, DNA replication, origin of replication, Pura, HIV-1 TAT, JC virus

The research program of Dr. Dianne C. Daniel addresses aspects of the cellular control of DNA replication and the DNA damage response.

DNA replication: In a recent collaborative effort, HIV-1 Tat protein and cellular protein Pura were shown to enhance replication from JC virus origin sequences in an in vitro replication assay as well as in vivo. Current studies are focused on our recently discovered MCM family member, MCM8, which may be involved in DNA replication control.

DNA damage response: The responses of human mammary basal myoepithelial and luminal epithelial cell types to DNA damage are being studied with a focus on BRCA1 and functionally related proteins. These studies are possible through the use of breast ductal and alveolar organoids prepared in the lab from tissue after reduction mammoplasty. Our research based on this model system indicates that the two differentiated cell types persist as long as the original organoid is present as opposed to cells serially passaged in culture, where a mixed phenotype has been observed. This research examines why most breast cancers are of the luminal phenotype.

Selected Publications

• Kinoshita Y, Johnson EM, Gordon RE, Negri-Bell H, Evans MT, Coolbaugh J, Rosario-Peralta Y, Samet J, Slusser E, Birkenbach MP, Daniel DC. Colocalization of MCM8 and MCM7 With Proteins Involved in Distinct Aspects of DNA Replication. Microsc Res Tech 2007 (DOI 10.1002/jemt.20553); 2008, 71(4):288-297.
   
• Daniel DC, Kinoshita Y, Khan MA, Valle LD, Khalili K, Rappaport J, Johnson EM. Internalization of exogenous human immunodeficiency virus-1 protein, Tat, by KG-1 oligodendroglioma cells followed by stimulation of DNA replication initiated at the JC virus origin. DNA Cell Biol 2004; 23(12):858-867.
   
• Johnson EM, Kinoshita Y, Daniel DC. A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13. Nucleic Acids Res 2003; 31(11):2915-2925.
   
• Daniel DC. Comment Article. BRCA1 and BRCA2 proteins in cell function. In: Wolvey W, editor. Breast Cancer. London, Current Medical Literature Ltd.; 2003. pp 1-9.
   
• Khalili K, Del Valle L, Muralidharan V, Gault WJ, Darbinian N, Otte J, Meier E, Johnson EM, Daniel DC, Kinoshita Y, Amini S, Gordon J. Pura is essential for postnatal brain development and developmentally-coupled cellular proliferation as revealed by genetic inactivation in the mouse. Mol Cell Biol 2003; 23(19):6857-6875.
   
• Daniel DC. Editorial. Breast cancer: cell biology, clinical parameters and imaging. Microsc Res Tech 2002; 59(1):1-2.
   
• Daniel DC. Highlight: BRCA1 and BRCA2 proteins in breast cancer. Microsc Res Tech 2002; 59(1):68-83.
   
• Daniel DC, Wortman MJ, Schiller RJ, Liu H, Gan L, Mellon JS, Chang CF, Gallia GL, Rappaport J, Khalili K, Johnson EM. Coordinate effects of human immunodeficiency virus type-1 protein, Tat, and cellular protein, Pur-alpha, on DNA replication initiated at the JC virus origin. J Gen Virol 2001; 82(Pt 7):1543-1553.
   
• Daniel DC. Dual immunofluorescence labeling with cell-specific markers localizes BRCA1 in both basal and luminal epithelial cells in primary outgrowth from noncancerous mammary ductal and alveolar preparations. Cell and Tissue Res 1999; 298(3):481-487.
   
• Daniel DC, Weston A. Benzo(a)pyrene enhances levels of ErbB2 in normal mammary epithelial cells in organoid culture. Cancer: Genetics and the Environment, Ann NY Acad Sci 1997; 833:169.
   
• Berberich S, Trivedi A, Daniel DC, Johnson EM, Leffak M. In Vitro Replication of Plasmids Containing Human c-myc DNA. J Mol Biol 1995; 245(2):92-109.

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