 Ann
E. Campbell, Ph.D.
Professor and Vice Chair
Lewis Hall, #3166
Office: (757) 446-5667
campbeae@evms.edu
Teaching: Virology and
Immunology
Biomedical Sciences
Program Track:
Molecular Integrative
Biosciences (MIB)
Education
-
B.S., University of
Maryland
-
M.S., Ph.D., Medical
College of Virginia
-
Postdoctoral Training,
Pennsylvania State University College of Medicine
Research Interests
Dr. Campbell's laboratory
pursues studies on the pathogenesis of the herpesvirus,
cytomegalovirus (CMV). This virus causes severe
complications in infected newborns, AIDS patients, and
immunosuppressed bone marrow or organ transplant recipients.
Acute and latent infections occur in a variety of organs and
cell types within the host.
One prominent cell type
infected during both acute and latent stages of infection is
the macrophage. This laboratory has identified a gene region
of murine CMV (MCMV) that is required for efficient
infection of macrophages and hence infectivity in vivo.
Deletion of three genes within this region results in a
mutant virus that replicates poorly in macrophages in vitro
and in mice. The product of these three genes has been
characterized, and they form a stable complex in infected
cells. By using a combination of genetic manipulations,
molecular biology, proteomics, and in vivo studies, the
laboratory pursues studies to characterize the structure of
this complex and to decipher the function of these viral
proteins in regulating cell tropism.
A second project aims to
identify the immune response to MCMV in the salivary gland,
a site of viral persistence. Assessment of the phenotype and
cytokine/chemokine profiles of cells infiltrating in
response to MCMV infection has identified populations of
cells unique to this mucosal site. Continued studies aim to
identify the function of the site-specific cells in
controlling infection.
Selected Publications
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Hanson, L.K., J.S. Slater,
Z. Karabekian, H.W. Virgin, C.A. Biron, M.C. Ruzek, N.
van Rooijen, R.P. Ciavarra, R.M. Stenberg, and A.E.
Campbell. 1999. Replication of murine
cytomegalovirus in differentiated macrophages as a
determinant of viral pathogenesis. J. Virol.
73:5970-5980.
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Hanson, L.K., B.L. Dalton,
H.E. Farrell, W.D. Rawlinson, R.M. Stenberg and A.E.
Campbell. 1999 .Transcriptional analysis of the
murine cytomegalovirus HindIII-I region: Identification
of a novel immediate early gene region. Virology
260:156-164.
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Hanson, L.K., J.S. Slater,
Z. Karabekian, G. Ciocco-Schmitt, and A.E. Campbell.
2001. Products of US22 gene M140 and M141 confer
efficient replication of murine cytomegalovirus in
macrophages and spleen. J. Virol. 75(14):6292-6302.
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Cavanaugh, V.J., Y. Deng, M.
Birkenbach, J.S. Slater and A.E. Campbell. 2002.
Vigorous innate and virus-specific CTL responses to
murine cytomegalovirus in the submaxillary salivary
gland. J. Virol. 77:1703-1717.
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Hanson, L.K., J.S. Slater,
Z. Karabekian, G. Ciocco-Schmitt, and A.E. Campbell.
2001. Products of US22 gene M140 and M141 confer
efficient replication of murine cytomegalovirus in
macrophages and spleen. J. Virol. 75(14):6292-6302.
-
Cavanaugh, V.J., Y. Deng, M.
Birkenbach, J.S. Slater and A.E. Campbell. 2002.
Vigorous innate and virus-specific CTL responses to
murine cytomegalovirus in the submaxillary salivary
gland. J. Virol. 77:1703-1717.
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Menard, C., M. Wagner, Z.
Ruszics, K. Holak, W. Brune, A.E. Campbell, and
U.H. Koszinowski. 2003. Role of murine cytomegalovirus
US22 gene family members for replication in macrophages.
J. Virol. 77:5557-5570.
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Karabekian, Z., L.K. Hanson,
J.S. Slater, N.K. Krishna, L.L. Bolin, J.A. Kerry, and
A.E. Campbell. 2005. Complex formation among murine
cytomegalovirus US22 proteins encoded by genes M139,
M140, and M141. J. Virol. 79:3525-3535.
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Hanson, L.K., L.F. Cageao,
R.E. Brock, J.S. Slater, B.L. Dalton, J.A. Kerry, and
A.E. Campbell. 2005. Characterization and regulation
of essential murine cytomegalovirus genes m142 and m143.
Virology 334:166-177.
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